EMBEDDING AID FOR COVERSLIP MOUNTS IN ELECTRON MICROSCOPY
نویسندگان
چکیده
منابع مشابه
Embedding Aid for Coverslip Mounts in Electron Microscopy
Borysko and Sapranauskas (1) and independently Gay (2) have described a method of making coverslip preparations of biological material from which small selected cell groups or single cells may be transferred to methacrylate for sectioning for electron microscopy. In both procedures it is suggested that the selected material viewed under light and phase microscopy be covered with a capsule conta...
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During an investigation of the effect of ischemia on striated muscle as seen with the electron microscope it became evident that all degrees of disorientation and destruction of tissues were taking place, and it seemed likely that much of the damage occurred during the embedding process. Such damage made it difficult to proceed with experiments in which comparison of normal tissue with any path...
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Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modifications to the conventional mixtures are the choice of a plasticized resin in conjunction with an alip...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1956
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.2.4.61